Thioredoxin Reductase Is Essential for Viability in the Fungal Pathogen Cryptococcus neoformans-Reference-Cited by-同舟云学术

Thioredoxin Reductase Is Essential for Viability in the Fungal Pathogen Cryptococcus neoformans

Published:2005-02 Issue:2 Volume:4 Page:487-489
ISSN:1535-9778
Container-title:Eukaryotic Cell
language:en
Short-container-title:Eukaryot Cell

Author:

Missall Tricia A.¹,Lodge Jennifer K.¹²

Affiliation:

1. Edward A. Doisy Department of Biochemistry and Molecular Biology

2. Department of Molecular Microbiology and Immunology, Saint Louis University School of Medicine, St. Louis, Missouri

Abstract

ABSTRACT Thioredoxin reductase ( TRR1 ) is an important component of the thioredoxin oxidative stress resistance pathway. Here we show that it is induced during oxidative and nitrosative stress and is preferentially localized to the mitochondria in Cryptococcus neoformans . The C. neoformans TRR1 gene encodes the low-molecular-weight isoform of the thioredoxin reductase enzyme, which shares little homology with that of its mammalian host. By replacing the endogenous TRR1 promoter with an inducible copper transporter promoter, we showed that Trr1 appears to be essential for viability of this pathogenic fungus, making it a potential antifungal target.

Publisher

American Society for Microbiology

Subject

Molecular Biology,General Medicine,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/EC.4.2.487-489.2005

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