New Diagnostic Real-Time PCR for Specific Detection of Parachlamydia acanthamoebae DNA in Clinical Samples-Reference-Cited by-同舟云学术

New Diagnostic Real-Time PCR for Specific Detection of Parachlamydia acanthamoebae DNA in Clinical Samples

Published:2008-04 Issue:4 Volume:46 Page:1491-1493
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Casson Nicola¹,Posfay-Barbe Klara M.²,Gervaix Alain²,Greub Gilbert¹

Affiliation:

1. Center for Research on Intracellular Bacteria, Institute of Microbiology, University Hospital Center and University of Lausanne, Lausanne, Switzerland

2. Children's Hospital of Geneva, University Hospitals of Geneva, Geneva, Switzerland

Abstract

ABSTRACT Given the low sensitivity of amoebal coculture, we developed a specific real-time PCR for the detection of Parachlamydia . The analytical sensitivity was high, and the inter- and intrarun variabilities were low. When the PCR was applied to nasopharyngeal aspirates, it was positive for six patients with bronchiolitis. Future studies should assess the role of Parachlamydia in bronchiolitis.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02302-07

Reference18 articles.

1. Obligate intracellular bacterial parasites of acanthamoebae related to Chlamydia spp

2. Chlamydia-like obligate parasite of free-living amoebae

3. Proc. 5th Annu. Workshop COST Action 855 Animal Chlamydioses Zoonotic Implications 2007

4. Casson, N., N. Medico, J. Bille, and G. Greub. 2006. Parachlamydia acanthamoebae enters and multiplies within pneumocytes and lung fibroblasts. Microbes Infect.8:1294-1300.

5. Casson, N., R. Michel, K. D. Muller, J. D. Aubert, and G. Greub. 2008. Protochlamydia naegleriophila as etiologic agent of pneumonia. Emerg. Infect. Dis.14:168-172.

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