Affiliation:
1. New England Eye Center, Boston, Massachusetts.
Abstract
Extensive immunohistochemical and thin-layer chromatogram-immunostain analyses were carried out to establish whether asialo GM1, a glycolipid which contains binding sites for Pseudomonas aeruginosa, is present in corneal epithelium. The data suggest that rabbit corneal epithelium does not contain detectable levels of asialo GM1 even after corneas are scarified and incubated with trypsin, P. aeruginosa, or P. aeruginosa exoproducts to expose potential cryptic sites. Preliminary immunohistochemical analyses indicated that asialo GM1 is also not found in human corneas.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference17 articles.
1. Baum J. and N. Panjwani. 1988. Adherence of Pseudomonas to soft contact lenses and corneas: mechanisms and prophylaxis p. 301-307. In H. D. Cavanagh (ed.) The cornea. Transactions of the World Congress on the Cornea III. Raven Press New York.
2. A simple method for the isolation and purification of total lipids from animal tissues;Folch J.;J. Biol. Chem.,1957
3. Evidence for asialo GM1 as a corneal glycolipid receptor for Pseudomonas aeruginosa adhesion;Hazlett L. D.;Infect. Immun.,1993
4. ~-Adrenergic and serotonergic responsiveness to rabbit corneal epithelial cells in culture;Jumblatt M. M.;Invest. Ophthalmol. Visual Sci.,1970
5. Animal glycosphingolipids as membrane attachment sites for bacteria. Annu;Karlsson K.;Rev. Biochem.,1989
Cited by
13 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献