Affiliation:
1. Northern Regional Research Laboratory, U.S. Department of Agriculture, Peoria, Illinois 61604
Abstract
Maximum yields of 1,250 IU (international unit)/g (dry weight of cells) of L-asparaginase were obtained in 8 hr from
Erwinia aroideae
NRRL B-138. Partial purification and concentration of the extracted L-asparaginase yielded a preparation with an activity of 275 IU/ml. Only one L-asparaginase was present as determined by electrophoresis, and the enzyme exhibited a
p
H optimum of 7.5 and a
K
m
of 3 × 10
-3
M.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
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