Polyclonal antibodies to chlorosome proteins as probes for green sulfur bacteria

Author:

Cahill A D1,Stolz J F1

Affiliation:

1. Department of Biological Sciences, Duquesne University, Pittsburgh, Pennsylvania 15282, USA.

Abstract

We found that polyclonal antibodies raised against chlorosome polypeptides from green sulfur bacteria reacted to Chlorobium tepidum, Chlorobium limicola, and Chlorobium phaeobacteroides but not to Chloroflexus aurantiacus. These antibodies successfully labeled only green sulfur species in marine microbial mat samples. Our results suggest that these antibodies may be useful as immunohistochemical probes.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference13 articles.

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2. D'Amelio E. D. Y. Cohen and D. J. Des Marais. 1989. Comparative functional ultrastructure of two hypersaline submerged microbial mats p. 97-113. In Y. Cohen and E. Rosenberg (ed.) Microbial mats: physiological ecology of benthic microbial communities. American Society for Microbiology Washington D.C.

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4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4;Laemmli U. K.;Nature (London),1970

5. Mack E. E. and B. K. Pierson. 1988. Preliminary characterization of temperate marine members of the Chloroflexaceae p. 237-241. In J. M. Olson J. G. Ormerod J. Amesz E. Stackebrandt and H. G. Trueper (ed.) Green photosynthetic bacteria. Plenum Press New York.

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