Direct detection of Listeria monocytogenes in 25 milliliters of raw milk by a two-step PCR with nested primers-Reference-Cited by-同舟云学术

Direct detection of Listeria monocytogenes in 25 milliliters of raw milk by a two-step PCR with nested primers

Published:1995-02 Issue:2 Volume:61 Page:817-819
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Herman L M¹,De Block J H¹,Moermans R J¹

Affiliation:

1. Government Dairy Research Station, Centre of Agricultural Research Ghent, Melle, Belgium.

Abstract

A method for direct detection of Listeria monocytogenes in 25 ml of raw milk is presented. The detection limit can be situated between 10 and 5 CFU. The detection method is based on chemical extraction of the milk components and PCR amplification with two nested pairs of primers specific for Listeria monocytogenes.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.61.2.817-819.1995

Reference18 articles.

1. Detection of viable Legionella pneumophila in water by polymerase chain reaction and gene probe methods;Bej A. K.;Appl. Environ. Microbiol.,1991

2. Detection of coliform bacteria in water by polymerase chain reaction and gene probes;Bej A. K.;Appl. Environ. Microbiol.,1990

3. Detection of Listeria species and Listeria monocytogenes using polymerase chain reaction;Border P. M.;Lett. Appl. Microbiol.,1990

4. Synthetic oligodeoxyribonucleotide probes for detection of Listeria monocytogenes;Datta A. R.;Appl. Environ. Microbiol.,1988

5. Entis P. 1986. Membrane filtration systems p. 91-106. In M. D. Pierson and N. J. Stern (ed.) Foodborne microorganisms and their toxins: developing methodology. Marcel Dekker New York.

Cited by 68 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Pulsed-field gel electrophoresis typing and molecular characterization of <em>Listeria monocytogenes</em> isolates in raw milk samples from Polonnaruwa District, Sri Lanka;Journal of the National Science Foundation of Sri Lanka;2023-10-12

2. Capacity building;Ensuring Global Food Safety;2022

3. Identification of Listeria monocytogenes in cattle meat using biochemical methods and amplification of the hemolysin gene;Anais da Academia Brasileira de Ciências;2020

4. Survival of a native toxigenic isolate of Listeria monocytogenes CFR 1302 during storage of milk-based foods can be a potential cause of health risk;Food Science and Technology International;2017-03-08

5. Short communication: Improved method for centrifugal recovery of bacteria from raw milk applied to sensitive real-time quantitative PCR detection of Salmonella spp.;Journal of Dairy Science;2016-05