Two-Year Study Evaluating the Potential Loss of Methicillin Resistance in a Methicillin-Resistant Staphylococcus aureus Culture Collection

Author:

Veguilla William1,Peak K. Kealy1,Luna Vicki A.1,Roberts Jill C.1,Davis Carisa R.1,Cannons Andrew C.1,Amuso Philip2,Cattani Jacqueline1

Affiliation:

1. Center for Biological Defense, College of Public Health, University of South Florida, Tampa, Florida

2. Florida Department of Health, Bureau of Laboratories, Tampa, Florida

Abstract

ABSTRACT A reported loss of mecA prompted us to monitor 360 cryostocked methicillin-resistant Staphylococcus aureus strains for stability. Concurrently, 14 well-characterized strains were stored in a Microbank preservation system and subjected to multiple freeze-thaw events. There were no significant declines in the methicillin-resistant populations with either method over a two-year period.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference16 articles.

1. Belt, A. 1996. Characterization of cultures used for biotechnology and industry, p. 251-258. In J. C. Hunter-Cevera and A. Belt (ed.), Maintaining cultures for biotechnology and industry. Academic Press, San Diego, CA.

2. Methicillin-resistant staphylococci

3. Clinical and Laboratory Standards Institute 2005

4. Clinical and Laboratory Standards Institute 2007

5. Reference deleted.

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