Abstract
The nucleotide sequences of 10 viable yet partially defective deletion mutants of simian virus 40 were determined. The deletions mapped within, and, in many cases, 5' to, the predominant leader sequence of the late viral mRNA's. They ranged from 74 to 187 nucleotide pairs in length. Six of the mutants had lost the sequence that corresponds to the "cap" site (5' terminus) of the most abundant class of 16S mRNA's. One of these mutants had a deletion that extended 103 nucleotide pairs into the region preceding this primary cap site and, therefore, was missing many secondary cap sites as well. A seventh mutant lacked the entire major 16S leader sequence except for the first six nucleotides at its 5' end and the last nine at its 3' end. Although these mutants differed in the size and position of their deletions, we were unable to discover any simple correlations between their growth characteristics and their DNA sequences. This finding indicates that the secondary structures of the RNA transcripts may play a more important role than the exact nucleotide sequence of the RNAs in determining how they function within the cell.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference31 articles.
1. Novel mechanism for RNA maturation: the leader sequences of simian virus 40 mRNA are not transcribed adjacent to the coding sequences;Aloni Y.;Proc. Natl. Acad. Sci. U.S.A.,1977
2. Spliced early mRNAs of simian virus 40;Berk A. J.;Proc. Natl. Acad. Sci. U.S.A.,1978
3. Ovalbumin gene: evidence for a leader sequence in mRNA and DNA sequences at the exon-intron boundaries;Breathnach R.;Proc. Natl. Acad. Sci. U.S.A.,1978
4. Comparison of the nucleotide sequence of the messenger RNA for the major structural proteins of SV40 with the DNA sequence encoding the amino acids of the protein;Celma M. L.;Nucleic Acids Res.,1977
5. Summary: the molecular biology of the eukaryotic genome is coming of age;Chambon P.;Cold Spring Harbor Symp. Quant. Biol.,1977
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