Affiliation:
1. Experimental Biology Research, Abbott Diagnostics Division, North Chicago, Illinois 60064.
Abstract
Amplification of Chlamydia trachomatis DNA by polymerase chain reaction was compared with amplification by ligase chain reaction (LCR). Both amplification procedures were able to consistently amplify amounts of DNA equivalent to three C. trachomatis elementary bodies. All 15 C. trachomatis serovars were amplified to detectable levels by LCR, and no DNA from 16 organisms potentially found in clinical specimens or from Chlamydia psittaci and Chlamydia pneumoniae was amplified by LCR.
Publisher
American Society for Microbiology
Cited by
93 articles.
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1. Chlamydia trachomatis (Trachoma, Genital Infections, Perinatal Infections, and Lymphogranuloma Venereum);Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases;2015
2. Molecular Diagnostics and Comparative Genomics in Clinical Microbiology;Molecular Diagnostics;2010
3. Chlamydia trachomatis (Trachoma, Perinatal Infections, Lymphogranuloma Venereum, and Other Genital Infections);Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases;2010
4. Epidemiology of Chlamydia trachomatis infection in women and the cost-effectiveness of screening;Human Reproduction Update;2009-10-14
5. Simultaneous Detection of Recombinant DNA Segments Introduced into Genetically Modified Crops with Multiplex Ligase Chain Reaction Coupled with Multiplex Polymerase Chain Reaction;Journal of Agricultural and Food Chemistry;2009-04-08