Detection of Cyclospora cayetanensis in Wastewater

Author:

Sturbaum Gregory D.1,Ortega Ynes R.2,Gilman Robert H.345,Sterling Charles R.2,Cabrera Lilia5,Klein Donald A.1

Affiliation:

1. Department of Microbiology, Colorado State University, Ft. Collins, Colorado1;

2. Department of Veterinary Science and Microbiology, University of Arizona, Tucson, Arizona2;

3. Department of International Health, Johns Hopkins University, Baltimore, Maryland3; and

4. Departamento de Patologia, Universidad Peruana Cayetano Heredia,4 and

5. Asociacion Benefica PRISMA,5 Lima, Peru

Abstract

ABSTRACT Cyclospora cayetanensis causes diarrheal disease worldwide without a confirmed mode of transmission. Wastewater was examined for the presence of this organism. Oocysts were detected microscopically, and their identity was confirmed by molecular techniques. These findings verify that current techniques can isolate Cyclospora oocysts and suggest that fecally contaminated water may act as a vehicle of transmission.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference8 articles.

1. American Public Health Association Immunofluorescence method for Giardia and Cryptosporidium spp. (PROPOSED) Standard methods for the examination of water and wastewater 19th ed. 1995 9 111 American Public Health Association Washington D.C

2. An outbreak in 1996 of cyclosporiasis associated with imported raspberries.;Herwaldt B. L.;N. Engl. J. Med.,1997

3. Jinneman K. C. Wetherington J. H. Adams A. M. Johnson J. M. Tenge B. J. Dang N.-L. Hill W. E. Differentiation of Cyclospora sp. and Eimeria spp. by using the polymerase chain reaction amplification products and restriction fragment length polymorphisms. Laboratory Information Bulletin No. 4044. U.S. 1996 Food and Drug Administration Washington D.C

4. Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples

5. A new coccidian parasite (Apicomplexa: Eimeriidae) from humans.;Ortega Y. R.;J. Parasitol.,1993

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