Estimation of Nitrifying Bacterial Activities by Measuring Oxygen Uptake in the Presence of the Metabolic Inhibitors Allylthiourea and Azide-Reference-Cited by-同舟云学术

Estimation of Nitrifying Bacterial Activities by Measuring Oxygen Uptake in the Presence of the Metabolic Inhibitors Allylthiourea and Azide

Published:1998-06 Issue:6 Volume:64 Page:2266-2268
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Ginestet Philippe¹,Audic Jean-Marc¹,Urbain Vincent¹,Block Jean-Claude²

Affiliation:

1. Centre International de Recherche sur l’Eau et l’Environment-Lyonnaise des Eaux, Le Pecq,1and

2. Laboratoire Santé-Environnement, UMR Université CNRS 7564, Faculté de Pharmacie, Nancy,2 France

Abstract

ABSTRACT The effects of two metabolic inhibitors on an enriched nitrifying biomass during incubation for short periods of time were investigated by determining respirometric measurements. Allylthiourea (86 μM) and azide (24 μM) were shown to be strong, selective inhibitors of ammonia and nitrite oxidation, respectively. Consequently, a differential respirometry method for estimating nitrifying and heterotrophic bacterial activities within a mixed biomass is proposed.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.64.6.2266-2268.1998

Reference19 articles.

1. Mechanism of nitrite oxidation and oxidoreductase systems in Nitrobacter agilis.;Aleem H. M. I.;Curr. Microbiol.,1981

2. Physiology, biochemistry, and specific inhibitors of CH4, NH4 +, and CO oxidation by methanotrophs and nitrifiers.;Bédard C.;Microbiol. Rev.,1989

3. Specific Inhibition of Nitrite Oxidation by Chlorate and Its Use in Assessing Nitrification in Soils and Sediments

4. Respirometric technique for determination of extant kinetic parameters describing biodegradation.;Ellis T. G.;Water Environ. Res.,1996

5. The enzymes associated with denitrification.;Hochstein L. I.;Annu. Rev. Microbiol.,1988

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