Affiliation:
1. Department of Microbiology, University of Barcelona, Avda. Diagonal 645, E-08028 Barcelona, Spain
Abstract
ABSTRACT
Many studies suggest a close relationship between species of
Bifidobacterium
and their hosts. Thus, species such as
B. adolescentis
and
B. thermacidophilum
subsp.
porcinum
have been proposed as potential indicators of human and porcine fecal pollution. The diversity of bifidobacteria in wastewaters (human and animal) and slurries is analyzed using nested PCR followed by denaturant gradient gel electrophoresis (DGGE). The sewage samples showed similar DGGE patterns. The predominant bands were recognized as
B. adolescentis
,
B. longum
, and two unidentified species related to
B. adolescentis
. A single band detected in poultry samples was identified as
B. saeculare
. Bifidobacterial diversity was higher within porcine and bovine samples. The main bands in porcine samples were identified as
B. minimum
, an unknown species, and
B. thermophilum/B. thermacidophilum
subsp.
porcinum
. The latter species was also identified among the main bands in bovine samples together with
B. pseudolongum
and
B. ruminantium
. We then attempted to isolate the host-specific strains. DGGE bands were examined to develop specific probes to screen environmental samples by colony hybridization and further isolation of strains from positively hybridized colonies. Bifidobacterial strains that are host associated by DGGE bands to human and pig were successfully isolated from the environment:
B. adolescentis
from human sewage samples and the unidentified species related to pig from slurries and slaughterhouse wastewater. Neither the poultry-associated
B. saeculare
nor the ruminant-associated
B. pseudolongum
could be isolated with the current methodology, suggesting either a low prevalence in the samples or failure of the culture to grow in the media used.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
24 articles.
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