The Synchrony Phenotype Persists after Elimination of Multiple GATC Sites from the dnaA Promoter of Escherichia coli-Reference-Cited by-同舟云学术

The Synchrony Phenotype Persists after Elimination of Multiple GATC Sites from the dnaA Promoter of Escherichia coli

Published:2006-06-15 Issue:12 Volume:188 Page:4573-4576
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Wilkinson Terry G.¹,Kedar G. C.¹,Lee Chi¹,Guzmán Elena C.²,Smith Douglas W.³,Zyskind Judith W.¹

Affiliation:

1. Department of Biology, San Diego State University, San Diego, California 92182-4614

2. Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Ciencias de Extremadura E-06080 Badajoz, Spain

3. Section of Molecular Biology, Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093-0348

Abstract

ABSTRACT To examine whether methylation of the GATC sites present in the dnaA promoter region is responsible for the strict temporal coordination of initiation events at oriC as measured by the synchrony of initiation, we introduced point mutations eliminating three (TGW1) and five (TGW2) of the six GATC sites present in the dnaA promoter region. All of the strains containing these mutations, including the one with five GATC sites eliminated, initiated chromosomal replication synchronously.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.00089-06

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