Detection of Chlamydia trachomatis in endocervical specimens by polymerase chain reaction

Author:

Loeffelholz M J1,Lewinski C A1,Silver S R1,Purohit A P1,Herman S A1,Buonagurio D A1,Dragon E A1

Affiliation:

1. Roche Molecular Systems, Branchburg, New Jersey 08876-1760.

Abstract

A rapid and sensitive polymerase chain reaction (PCR)-based assay for detection of Chlamydia trachomatis in cervical specimens is described. This assay consists of (i) sample preparation which avoids the use of heat, centrifugation, or organic extractions; (ii) rapid, two-temperature PCR amplification of C. trachomatis cryptic plasmid sequences; and (iii) capture and colorimetric detection of amplified DNA in microwell plates. PCR was compared with culture by using 503 cervical specimens. After resolution of discrepant specimens with a confirmatory PCR assay directed against the chlamydial major outer membrane protein gene, PCR had a sensitivity of 97% and a specificity of 99.7% while culture had a sensitivity of 85.7% and a specificity of 100%. In a separate study, PCR was compared with a direct specimen enzyme immunoassay (Chlamydiazyme; Abbott Diagnostics) by using 375 cervical specimens. After resolution of discrepant specimens, PCR had a sensitivity and specificity of 100%, while the enzyme immunoassay had a sensitivity of 58.8% and a specificity of 100%.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference17 articles.

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2. Diagnosis of Chlamydia trachomatis cervical infection by detection of amplified DNA with an enzyme immunoassay;Bobo L.;J. Clin. Microbiol.,1990

3. Chlamydia trachomatis infections, policy guidelines for prevention and control;Centers for Disease Control.;Morbid. Mortal. Weekly Rep.,1985

4. Diagnostic value of the polymerase chain reaction for Chlamydia detection as determined in a follow-up study;Class H. C. J.;J. Clin. Microbiol.,1991

5. Diversity of the chlamydial common plasmid in biovars with different pathogenicity;Comanducci M.;Plasmid,1990

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