Powdered Chitin Agar as a Selective Medium for Enumeration of Actinomycetes in Water and Soil

Author:

Hsu S. C.1,Lockwood J. L.1

Affiliation:

1. Bureau of Laboratories, Michigan Department of Public Health, Lansing, Michigan 48914, and Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824

Abstract

Agar media made with 0.4% colloidal chitin plus mineral salts and adjusted to pH 8.0 was superior to four other commonly used media for the isolation and enumeration of actinomycetes from water samples. More actinomycetes developed on chitin agar, and the development of bacteria and fungi was suppressed. Frozen and vacuum-dried chitin from aqueous colloidal suspensions was finely divided and gave results comparable to those obtained with media prepared from colloidal suspensions. Images

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference15 articles.

1. The selective enumeration of actinomycetes in the presence of large numbers of fungi;Corke C. T.;Can. J. Microbiol.,1956

2. The use of sodium propionate in isolating actinomycetes from soil;Crook P.;Science,1950

3. A note on the isolation of microorganisms from natural sources;Dulaney E. L.;Mycologia,1955

4. Difco Laboratories. 1962. Bacto-actinomycete isolation agar. Code 0957 Difco Supplementary Literature. Difco Laboratories Detroit Mich.

5. Selective isolation of aerobic actinomycetes;El-Nakeeb M. A.;Appl. Microbiol.,1963

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