Rubella Hemagglutination Inhibition: Removal of Nonspecific Agglutination Due to Manganous Chloride-Reference-Cited by-同舟云学术

Rubella Hemagglutination Inhibition: Removal of Nonspecific Agglutination Due to Manganous Chloride

Published:1974-12 Issue:6 Volume:28 Page:992-994
ISSN:0003-6919
Container-title:Applied Microbiology
language:en
Short-container-title:Appl Microbiol

Author:

Biano S. A.¹,Chang Te Wen¹,Daniels J. B.¹

Affiliation:

1. Boston Hospital for Women, Harvard Medical School, and New England Medical Center, Tufts University Medical School, Boston, Massachusetts 02115; and Cape Cod Hospital, Harvard Medical School, Hyannis, Massachusetts 02601

Abstract

Nonspecific inhibitors of rubella hemagglutination can be removed by treatment of sera with heparin-manganous chloride for use in the hemagglutination-inhibition test. After removal of nonspecific inhibitors by this procedure, an excess of manganous chloride may remain. This may cause the cells to agglutinate, thus obscuring the reading at low serum dilutions. This disadvantage can be overcome by the addition of sodium carbonate, which forms an insoluble compound with manganous chloride and does not interfere with antibody determination. The procedure presents a further refinement of the hemagglutination inhibition test for rubella by increasing specificity and sensitivity; it permits detection of antibody levels as low as 1:4 in sera.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://journals.asm.org/doi/pdf/10.1128/am.28.6.992-994.1974

Reference6 articles.

1. Rubella antibodies in human serum; detection by the indirect fluorescent antibody technic;Brown G. C.;Science,1964

2. On the precipitation of P-lipoprotein of the serum by heparin in the presence of certain bivalent cations;Burstein M.;C. R. Acad. Sci. Clin. Physiol.,1967

3. Cremer N. E. 1974. Immunodiagnosis of rubella virus infection. Public Health Laboratory report p. 87-97. Conference of Public Health Laboratory Directors Pierre S.D.

4. The nonspecific inhibitors of rubella virus hemagglutination;Dold H. J.;Proc. Soc. Exp. Biol. Med.,1968

5. Rubella-virus hemagglutination inhibition test;Stewart G. L.;N. Engl. J. Med.,1967

Cited by 2 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Rubella hemagglutination-inhibition test: false-positive reactions in sera contaminated with bacteria;Journal of Clinical Microbiology;1976-11

2. Simple procedure for the removal of nonspecific inhibitors of rubella virus hemagglutination;Journal of Clinical Microbiology;1975-12