Author:
Engelberg-Kulka H,Israeli-Reches M,Dekel L,Friedmann A
Abstract
This paper describes Q beta noninfectious particles produced at 41 degrees C in a streptomycin-resistant Escherichia coli mutant which is temperature sensitive for suppression of a nonsense codon. The noninfectious particles resembled Q beta under the electron microscope and contained coat protein molecules in an amount similar to the amount in Q beta. However, they did not adsorb to F-piliated bacteria, and they were deficient in both minor capsid proteins of Q beta, maturation (IIa) and read-through (IIb). Proteins IIa and IIb were not produced in Qbeta-infected mutant cells at 41 degrees C. In addition, instead of the 30S RNA of Q beta, a shorter RNA, which sedimented mainly at 23 S, was found in the defective particles. The results are discussed in relation to the roles of proteins IIa and IIb of Q beta.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference41 articles.
1. Adams M. H. 1959. Assay of phage by agar layer methods p. 450-451. In Bacteriophages. Intersciences Publishers Inc. New York.
2. Intact ribonucleic acid f'rom defective particles of bacteriophage R17;Argetsinger J. E.;J. Mol. Biol.,1966
3. Conformation independent molecular weight determination of RNA by gel electrophoresis;Boedtker H.;Biochim. Biohvs. Acta,1971
4. Suppression of UTGA codon by tryptophan tRNA;Chan T. S.;J. Mol. Biol.,1971
5. High pleiotrophy of streptomrycin mutation Escherichia coti;Couturier M.;Biochem. Biophys. Res. Commun.,1964
Cited by
14 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献