Affiliation:
1. Department of Medical Biotechnology, Flinders University, Bedford Park, South Australia 5042, Australia
Abstract
ABSTRACT
This is the first report of filamentous actinobacteria isolated from surface-sterilized root tissues of healthy wheat plants (
Triticum aestivum
L.). Wheat roots from a range of sites across South Australia were used as the source material for the isolation of the endophytic actinobacteria. Roots were surface-sterilized by using ethanol and sodium hypochlorite prior to the isolation of the actinobacteria. Forty-nine of these isolates were identified by using 16S ribosomal DNA (rDNA) sequencing and found to belong to a small group of actinobacterial genera including
Streptomyces
,
Microbispora
,
Micromonospora
, and
Nocardiodes
spp. Many of the
Streptomyces
spp. were found to be similar, on the basis of their 16S rDNA gene sequence, to
Streptomyces
spp. that had been isolated from potato scabs. In particular, several isolates exhibited high 16S rDNA gene sequence homology to
Streptomyces caviscabies
and
S. setonii
. None of these isolates, nor the
S. caviscabies
and
S. setonii
type strains, were found to carry the
nec1
pathogenicity-associated gene or to produce the toxin thaxtomin, indicating that they were nonpathogenic. These isolates were recovered from healthy plants over a range of geographically and temporally isolated sampling events and constitute an important plant-microbe interaction.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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