Affiliation:
1. Department of Molecular Biology, Hebrew University, Hadassah Medical School, Jerusalem, Israel
Abstract
A new radiometric method for the detection of coliform bacteria in water has been described. The method is based on the release of
14
CO
2
from [
14
C]lactose by bacteria suspended in growth medium and incubated at 37 C. The evolved
14
CO
2
is trapped by hyamine hydroxide and counted in a liquid scintillation spectrometer. The method permits the detection of 1 to 10 organisms within 6 h of incubation. Coliform bacteria suspended in water for several days recover from starvation and may be quantitated by the proposed method. Bacteria from water samples may also be concentrated by filtration through membrane filters and detected by the radiometric assay.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Cited by
7 articles.
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