Affiliation:
1. Michigan Department of Health Laboratories, Grand Rapids, and the School of Public Health, The University of Michigan, Ann Arbor, Michigan
Abstract
Eldering, Grace
(Michigan Department of Health, Grand Rapids),
Warren C. Eveland, and Pearl L. Kendrick
. Fluorescent antibody staining and agglutination reactions in
Bordetella pertussis
cultures. J. Bacteriol.
83:
745–749. 1962—
Bordetella pertussis
antisera produced with smooth cultures gave positive results in agglutination and fluorescent antibody (FA) tests with smooth
B. pertussis
cultures. Similar results were obtained with antisera produced with the related species
B. parapertussis
and
B. bronchiseptica
when each was tested with the respective homologous antigen. Cross reactions occurred with some of the antisera and heterologous antigens but a positive agglutination test was not always correlated with a positive FA reaction, and the reverse was also observed. Adsorbed
B. pertussis
antisera specific for factors 2, 3, 4, and 5 agglutinated appropriate
B. pertussis
cultures, but gave negative FA reactions. Factor 1 antiserum prepared by adsorption with heated (100 C) antigen gave positive results in both FA and agglutination tests. Rough
B. pertussis
cultures were inagglutinable, but were FA positive when tested with unadsorbed antiserum produced with either smooth or rough cultures. Rough cultures were FA negative with factor 1 serum. The results suggest that for
B. pertussis
different serum components may be responsible for agglutination and FA staining. The implications with respect to the protection-inducing antigen are discussed.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference10 articles.
1. Serological studies on Haemophilus pertussis, Haemophilus parapertussis, and Haemophilus bronchiseptica;ANDERSEN E. K.;Acta Pathol. Microbiol. Scand.,1953
2. The failure to show correlation between type-specificity and protection in experimental pertussis in mice;ANDERSEN E. K.;Acta Pathol. Microbiol. Scand.,1958
3. Diagnosis of pertussis by fluorescent antibody staining of nasopharyngeal smears;DONALDSON P.;Am. J. Diseases Children,1960
4. Serological study of Bordetella pertussis and related species;ELDERING G., C.;J. Bacteriol.,1957
5. Fluorescent antibody techniques;KENDRICK P. L.;Am. J. Diseases Children,1961
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