Affiliation:
1. Laboratory Division, National Communicable Disease Center, Atlanta, Georgia 30333
Abstract
Standardized hemagglutination and hemagglutination-inhibition procedures are described and statistically evaluated for all animal viruses where applicable, except for rubella and the arbovirus group. The standardized tests employ a constant phosphate-buffered saline diluent and constant volumes of serum, antigen, and standardized erythrocyte suspension. The standardized hemagglutination test has a reproducibility of 84 to 96% with adenoviruses, rubeola, and the myxoviruses, and 78 to 93% with reoviruses; the standardized hemagglutination-inhibition test has a reproducibility of 95 to 100% with all viruses tested.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Reference31 articles.
1. Casals J. 1967. Immunological techniques for animal viruses p. 163-174. In K. Maramorosch and H. Koprowski (ed.) Methods in virology vol. 3. Academic Press Inc. New York.
2. Photometric methods for the measurement of hemagglutinating viruses and antibody. I. Further experience with a novel photometric method for measuring hemagglutinins;Drescher J.;J. Immunol.,1962
3. Agglutination of bovine erythrocytes: a general characteristic of reovirus type 3;Eggers H. J.;Proc. Soc. Exp. Biol. Med.,1962
4. Routine HA and HAI tests for identifying enterovirus and reovirus strains;Feorino P. M.;Public Health Rep.,1963
5. Hierholzer J. C. and M. T. Suggs. 1969. Standardized viral hemagglutination and hemagglutination-inhibition tests. Standardization of erythrocyte suspensions. Appl.
Cited by
62 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献