Cloning and Characterization of a Novel Trimethoprim-Resistant Dihydrofolate Reductase from a Nosocomial Isolate of Staphylococcus aureus CM.S2 (IMCJ1454)-Reference-Cited by-同舟云学术

Cloning and Characterization of a Novel Trimethoprim-Resistant Dihydrofolate Reductase from a Nosocomial Isolate of Staphylococcus aureus CM.S2 (IMCJ1454)

Published:2005-09 Issue:9 Volume:49 Page:3948-3951
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Sekiguchi Jun-ichiro¹,Tharavichitkul Prasit²,Miyoshi-Akiyama Tohru¹,Chupia Vena²,Fujino Tomoko¹,Araake Minako¹,Irie Atsushi¹,Morita Koji³,Kuratsuji Tadatoshi⁴⁵,Kirikae Teruo¹

Affiliation:

1. Department of Infectious Diseases

2. Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai 50200, Thailand

3. Department of Microbiology, Kyorin University School of Health Sciences, Hachioji, Tokyo 192-8508, Japan

4. Research Institute, International Medical Center of Japan, Shinjuku, Tokyo 162-8655, Japan

5. National Research Institute for Child Health and Development, Setagaya, Tokyo 157-8535, Japan

Abstract

ABSTRACT A novel gene, dfrG , encoding a trimethoprim (TMP)-resistant dihydrofolate reductase (DHFR, designated S3DHFR) was cloned from a clinical isolate of methicillin-resistant Staphylococcus aureus. Escherichia coli expressing dfrG was highly resistant to TMP. Recombinant S3DHFR exhibited DHFR activity that was not inhibited by TMP.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.49.9.3948-3951.2005

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