Development of a New Serological Test for Serotyping Haemophilus parasuis Isolates and Determination of Their Prevalence in North America

Author:

Tadjine M.1,Mittal K. R.1,Bourdon S.1,Gottschalk M.1

Affiliation:

1. Département de Pathologie et Microbiologie, Faculté de Médecine Vétérinaire, Université de Montréal, Sainte-Hyacinthe, Québec, Canada J2S 7C6

Abstract

ABSTRACT Haemophilus parasuis causes polyserositis in swine. Fifteen serovars have been characterized by immunodiffusion test, but many field strains are not typeable. Isolates ( n = 300) of H. parasuis from animals in North America were serotyped by a new indirect hemagglutination test. The test was rapid and effective for serotyping of H. parasuis , and serovars 4, 5, 13, and 7 were the most prevalent serotypes.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference18 articles.

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2. Borduas, A. G., and P. Grabar. 1953. L'hémagglutination passive dans la recherche des anticorps antiprotéinique. Ann. Inst. Pasteur84:903-923.

3. Boyden, S. V. 1951. The adsorption of proteins on erythrocytes treated with tannic acid, and subsequent hemagglutination with antiprotein sera. J. Exp. Med.93:107-110.

4. Kielstein, P., and A. Raβbach. 1991. Serologische typisierung and nachweis immunogener kreuzreaktionen von Hemophilus parasuis (Glässersche Krankheit). Monatsh. Vetmed.46:586-589.

5. Kielstein, P., H. Rosner, and W. Müller. 1991. Typing of heat-stable soluble Haemophilus parasuis antigen by means of agar gel precipitation and the dot-blot procedure. J. Vet. Med.8:315-320.

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