Evaluation of the RealArt Malaria LC Real-Time PCR Assay for Malaria Diagnosis-Reference-Cited by-同舟云学术

Evaluation of the RealArt Malaria LC Real-Time PCR Assay for Malaria Diagnosis

Published:2004-02 Issue:2 Volume:42 Page:636-638
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Farcas Gabriella A.¹,Zhong Kathleen J. Y.²,Mazzulli Tony³⁴,Kain Kevin C.¹⁵²⁴

Affiliation:

1. Institute of Medical Science, Department of Medicine

2. Tropical Disease Unit, Division of Infectious Diseases, Toronto General Hospital-University Health Network

3. Department of Laboratory Medicine and Pathobiology

4. Department of Microbiology, Toronto Medical Labs/Mount Sinai Hospital, Toronto, Ontario, Canada

5. Global Health Program, McLaughlin Center for Molecular Medicine, University of Toronto

Abstract

ABSTRACT PCR-based methods have advantages over traditional microscopic methods for the diagnosis of malaria, especially in cases of low parasitemia and mixed infections. However, current PCR-based assays are often labor-intensive and not readily quantifiable and have the potential for contamination due to a requirement for postamplification sample handling. Real-time PCR can address these limitations. This study evaluated the performance characteristics of a commercial malaria real-time PCR assay (RealArt Malaria LC Assay; Artus GmbH, Hamburg, Germany) on the LightCycler platform for the detection of malaria parasites in 259 febrile returned travelers. Compared to nested PCR as the reference standard, the real-time assay had a sensitivity of 99.5%, specificity of 100%, positive predictive value of 100%, and negative predictive value of 99.6% for the detection of malaria. Our results indicate that the RealArt assay is a rapid (<45 min), sensitive, and specific method for the detection of malaria in returned travelers.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.42.2.636-638.2004

Reference18 articles.

1. Blair, P. L., A. Witney, J. D. Haynes, J. K. Moch, D. J. Carucci, and J. H. Adams. 2002. Transcripts of developmentally regulated Plasmodium falciparum genes quantified by real-time RT-PCR. Nucleic Acids Res.30:2224-2231.

2. Brown, A. E., K. C. Kain, J. Pipithkul, and H. K. Webster. 1992. Demonstration by the polymerase chain reaction of mixed Plasmodium falciparum and P. vivax infections undetected by conventional microscopy. Trans. R. Soc. Trop. Med. Hyg.86:609-612.

3. Bruna-Romero, O., J. C. R. Hafalla, G. Gonzalez-Aseguinolaza, G. Sano, M. Tsuji, and F. Zavala. 2001. Detection of malaria liver-stage in mice infected through the bite of a single Anopheles mosquito using a highly sensitive real-time PCR. Int. J. Parasitol.31:1499-1502.

4. Fabre, R., A. Berry, and J. F. Magnaval. 2003. Diagnosis of imported malaria with a multiplex PCR on LightCycler apparatus. Pathol. Biol. (Paris)51:44-46.

5. Hanscheid, T., and M. P. Grobusch. 2002. How useful is PCR in the diagnosis of malaria? Trends Parasitol.18:395-398.

Cited by 61 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. EVALUATION OF PERFORMANCE OF TWO MONOPLEX QUANTITATIVE REAL TIME PCR ASSAYS IN COMPARISON TO MICROSCOPY FOR IDENTIFICATION OF MALARIA PARASITES.;PARIPEX INDIAN JOURNAL OF RESEARCH;2022-03-15

2. A Rapid FRET Real-Time PCR Protocol for Simultaneous Quantitative Detection and Discrimination of Human Plasmodium Parasites;BIO-PROTOCOL;2022

3. Diagnostic Methods for Non-Falciparum Malaria;Frontiers in Cellular and Infection Microbiology;2021-06-17

4. Validation of a novel FRET real-time PCR assay for simultaneous quantitative detection and discrimination of human Plasmodium parasites;PLOS ONE;2021-06-04

5. Diagnosis of clinical malaria in endemic settings;Expert Review of Anti-infective Therapy;2020-08-30