Affiliation:
1. Department of Plant Pathology, New York State Agricultural Experiment Station, Cornell University, Geneva, New York 14456
Abstract
Probes consisting of T-DNA genes from the Ti plasmid of
Agrobacterium tumefaciens
were used for determining tumorigenicity of strains. Two
32
P-labeled probes hybridized with 28 of 28 tumorigenic strains of the pathogen but not with 20 of 22 nontumorigenic strains. One probe, pTHE17, consists of all but the far left portion of the T-DNA of strain C58. Probe
Sma
I7 consists of
Sma
I fragment 7 of pTiC58, including
onc
genes 1, 4, and 6a and most of 2. Another probe, pAL4044, consisting of the
vir
region of strain Ach-5, hybridized with several nontumorigenic as well as tumorigenic strains. Colony hybridizations were done with 28 tumorigenic and 22 nontumorigenic
Agrobacterium
strains. About 10
6
CFU of the different tumorigenic strains were detectable with this method. Southern analyses confirmed the presence or absence of Ti plasmids in strains for which tumorigenicity was questioned. Colony hybridization with the T-DNA probes provides a rapid and sensitive means for determining the tumorigenic nature of
Agrobacterium
strains.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
16 articles.
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