Affiliation:
1. Department of Tumor Biology, Karolinska Institute, Stockholm, Sweden.
Abstract
We have analyzed the expression of the three major known growth transformation-associated Epstein-Barr virus (EBV) proteins, EBNA-1, EBNA-2, and latent membrane protein (LMP), in a series of somatic cell hybrids derived from the fusion of EBV-carrying Burkitt lymphoma (BL) lines with EBV-positive or EBV-negative B-cell lines. Independently of the cell phenotype, EBNA-1 was invariably coexpressed in all EBV-carrying hybrids. In hybrids between EBV-carrying, LMP-positive and LMP-negative Burkitt lymphoma lines, LMP was expressed, indicating positive control. Two EBV-negative lymphoma lines, Ramos and BJAB, differed in their ability to express LMP after B95-8 virus-induced conversion and after hybridization with Raji cells. BJAB was permissive while Ramos was nonpermissive for LMP, although both expressed EBNA-2. The EBNA-2-deleted P3HR-1 virus gave the same pattern of LMP expression in these two cells. Our findings indicate that the expression of EBNA-1, EBNA-2, and LMP is regulated by independent mechanisms.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference49 articles.
1. Transformation of Balb 3T3 cells by the BNLF-1 gene of Epstein-Barr virus;Baichwald V. R.;Oncogene,1988
2. Epstein-Barr virus (EBV) induces expression of B-cell activation markers on in vitro infection of EBV-negative B-lymphoma cells;Calender A.;Proc. Natl. Acad. Sci. USA,1987
3. Selection of biochemically variant clones of human Iymphoid cells;Clements G. B.;Ann. Hum. Genet.,1975
4. Production by EBV infection of an EBNA-positive subline from an EBNAnegative human Iymphoma cell line without detectable EBV DNA;Clements G. B.;Int. J. Cancer,1975
5. Heterogeneity of Epstein-Barr virus. III. Comparison of a transforming and nontransforming virus by partial denaturation mapping of their DNAs;Delius H.;J. Virol.,1978
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