Affiliation:
1. Institute of Microbial Technology, Chandigarh
2. National Institute of Cholera and Enteric Diseases, Calcutta, India
3. International Centre for Diarrhoeal Disease Research, Dhaka 1000, Bangladesh
Abstract
ABSTRACT
Molecular mechanisms of multidrug resistance in
Vibrio cholerae
belonging to non-O1, non-O139 serogroups isolated during 1997 to 1998 in Calcutta, India, were investigated. Out of the 94 strains examined, 22 strains were found to have class I integrons. The gene cassettes identified were
dfrA1
,
dfrA15
,
dfrA5
, and
dfrA12
for trimethoprim;
aac
(
6
′)
-Ib
for amikacin and tobramycin;
aadA1
and
aadA2
for streptomycin and spectinomycin; and
ereA2
for erythromycin resistance. To our knowledge, this is the first report of the presence of
dfrA5
,
dfrA12
,
aac
(
6
′)
-Ib,
and
ereA2
cassettes in class I integrons of
V. cholerae
. Forty-three of 94 strains also had plasmids, and out of these, 14 contained both class I integrons and plasmids. Pulsed-field gel electrophoresis followed by Southern hybridization revealed that in the 14 plasmid-bearing strains, class I integrons resided either on chromosomes, on plasmids, or on both. Our results indicated that besides class I integrons and plasmids, a conjugative transposon element, SXT, possibly contributed to the multiple antibiotic resistance.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Cited by
133 articles.
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