Comparison of the BD GeneOhm Methicillin-Resistant Staphylococcus aureus (MRSA) PCR Assay to Culture by Use of BBL CHROMagar MRSA for Detection of MRSA in Nasal Surveillance Cultures from an At-Risk Community Population

Author:

Farley Jason E.12,Stamper Paul D.3,Ross Tracy3,Cai Mian3,Speser Sharon3,Carroll Karen C.243

Affiliation:

1. The Johns Hopkins University School of Nursing

2. Division of Infectious Disease

3. Microbiology Laboratory, Johns Hopkins Hospital, Baltimore, Maryland

4. Division of Medical Microbiology, Department of Pathology, The Johns Hopkins University School of Medicine

Abstract

ABSTRACT We compared the BD GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) PCR assay to culture with BBL CHROMagar MRSA for nasal surveillance among 602 arrestees from the Baltimore City Jail. The sensitivity and specificity were 88.5% and 91.0%, respectively, and after secondary analysis using enrichment broth, they were 89.0% and 91.7%, respectively. Twenty-three of 42 false-positive PCR lysates contained methicillin-susceptible S. aureus .

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference24 articles.

1. Concurrent Analysis of Nose and Groin Swab Specimens by the IDI-MRSA PCR Assay Is Comparable to Analysis by Individual-Specimen PCR and Routine Culture Assays for Detection of Colonization by Methicillin-Resistant Staphylococcus aureus

2. Evaluation of the BD Phoenix Automated Microbiology System for Identification and Antimicrobial Susceptibility Testing of Staphylococci and Enterococci

3. Centers for Disease Control and Prevention. 2003. Methicillin-resistant Staphylococcus aureus infections in correctional facilities—Georgia, California and Texas, 2001-2003. MMWR Morb. Mortal Wkly. Rep.52:992-996.

4. Centers for Disease Control and Prevention. 2003. Outbreaks of community-associated MRSA skin infections—Los Angeles County, California, 2002-2003. MMWR Morb. Mortal Wkly. Rep.52:793-795.

5. Centers for Disease Control and Prevention. 2001. Methicillin-resistant Staphylococcus aureus skin or soft tissue infections in a state prison—Mississippi, 2000. MMWR Morb. Mortal Wkly. Rep.50:919-922.

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