Identification of Variable-Number Tandem-Repeat (VNTR) Sequences in Legionella pneumophila and Development of an Optimized Multiple-Locus VNTR Analysis Typing Scheme-Reference-Cited by-同舟云学术

Identification of Variable-Number Tandem-Repeat (VNTR) Sequences in Legionella pneumophila and Development of an Optimized Multiple-Locus VNTR Analysis Typing Scheme

Published:2007-04 Issue:4 Volume:45 Page:1190-1199
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Pourcel Christine¹,Visca Paolo²³,Afshar Baharak⁴,D'Arezzo Silvia²,Vergnaud Gilles¹⁵,Fry Norman K.⁴

Affiliation:

1. Univ Paris-Sud, Institut de Génétique et Microbiologie, CNRS, Orsay F-91405, France

2. Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani I.R.C.C.S

3. Dipartimento di Biologia, Università Roma Tre, Rome, Italy

4. Health Protection Agency, Respiratory and Systemic Infection Laboratory, Centre for Infections, London, United Kingdom

5. Division de Microbiologie Analytique, Centre d'Etudes du Bouchet, Vert le Petit, France

Abstract

ABSTRACT The utility of a genotypic typing assay for Legionella pneumophila was investigated. A multiple-locus variable number of tandem repeats (VNTR) analysis (MLVA) scheme using PCR and agarose gel electrophoresis is proposed based on eight minisatellite markers. Panels of well-characterized strains were examined in a multicenter analysis to validate the assay and to compare its performance to that of other genotyping assays. Excellent typeability, reproducibility, stability, and epidemiological concordance were observed. The MLVA type or profile is composed of a string of allele numbers, corresponding to the number of repeats at each VNTR locus, separated by commas, in a predetermined order. A database containing information from 99 L. pneumophila serogroup 1 strains and four strains of other serogroups and their MLVA profiles, which can be queried online, is available from http://bacterial-genotyping.igmors.u-psud.fr/ .

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02078-06

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