Inducible CRISPRi-Based Operon Silencing and Selective in <i>Trans</i> Gene Complementation in Borrelia burgdorferi-Reference-Cited by-同舟云学术

Inducible CRISPRi-Based Operon Silencing and Selective in Trans Gene Complementation in Borrelia burgdorferi

Published:2023-02-22 Issue:2 Volume:205 Page:
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Murphy Bryan T.¹,Wiepen Jacob J.¹,He Huan¹,Pramanik Ankita S.¹,Peters Jason M.²^ORCID,Stevenson Brian³^ORCID,Zückert Wolfram R.¹^ORCID

Affiliation:

1. University of Kansas School of Medicine, Department of Microbiology, Molecular Genetics and Immunology, Kansas City, Kansas, USA

2. University of Wisconsin-Madison, School of Pharmacy, Pharmaceutical Sciences Division, Madison, Wisconsin, USA

3. University of Kentucky College of Medicine, Department of Microbiology, Immunology and Molecular Genetics, Lexington, Kentucky, USA

Abstract

Borrelia burgdorferi , the spirochetal bacterium causing Lyme disease, is a tick-borne pathogen of global importance. Here, we expand the genetic toolbox for studying B. burgdorferi physiology and pathogenesis by establishing a single plasmid-based, fully inducible, and nontoxic CRISPR interference (CRISPRi) system for transcriptional silencing of B. burgdorferi genes and operons.

Funder

KUMC Lied Basic Science Pilot Grant

KUMC Biosciences Research Training Grant

HHS | NIH | National Institute of Allergy and Infectious Diseases

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.00468-22

Reference74 articles.

1. The next generation of CRISPR–Cas technologies and applications