Temperature-Dependent Fermentation of d -Sorbitol in Escherichia coli O157:H7

Author:

Bouvet O. M. M.1,Pernoud S.1,Grimont P. A. D.1

Affiliation:

1. Unité des Entérobactéries, Unité INSERM 389, Institut Pasteur, 75724 Paris Cedex 15, France

Abstract

ABSTRACT The influence of growth temperature on the ability to ferment d -sorbitol was investigated in Escherichia coli O157:H7. It was found that O157:H7 strains have a temperature-sensitive sorbitol phenotype. d -Sorbitol transport and sorbitol-6-phosphate dehydrogenase activities were expressed in sorbitol-fermenting cells grown at 30°C but only at a low level at 40°C. Sorbitol-positive variants able to transport d -sorbitol were easily selected at 30°C from culture of Sor E. coli O157:H7 strains.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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