Biophysical and Biochemical Studies on Rhinovirus and Poliovirus II. Chemical and Hydrodynamic Analysis of the Rhinovirion-Reference-Cited by-同舟云学术

Biophysical and Biochemical Studies on Rhinovirus and Poliovirus II. Chemical and Hydrodynamic Analysis of the Rhinovirion

Published:1971-01 Issue:1 Volume:7 Page:41-46
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

McGregor Sandy¹,Mayor Heather D.¹

Affiliation:

1. WHO International Reference Centre for Enteroviruses, Department of Virology and Epidemiology, Baylor College of Medicine, Houston, Texas 77025

Abstract

Chemical analysis of rhinovirus 14 revealed a ribonucleic acid (RNA) content of 29.8% and a high adenylic acid content (35%). A partial specific volume of 0.682 cm 3 /g was obtained for the rhinovirion. Rhinovirus and poliovirus had identical sedimentation coefficients of 158 S. A diffusion coefficient of 1.71 × 10 −7 cm 2 /sec was consistent with a hydrated diameter of 25 nm for the rhinovirion. The calculated molecular weights of the rhinovirion and its genome were 7.1 × 10 6 and 2.1 × 10 6 daltons, respectively. Sedimentation analysis of infectious RNA confirmed the similarity of the molecular size of the poliovirus and rhinovirus genomes.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.7.1.41-46.1971

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