African 2, a Clonal Complex of Mycobacterium bovis Epidemiologically Important in East Africa-Reference-Cited by-同舟云学术

African 2, a Clonal Complex of Mycobacterium bovis Epidemiologically Important in East Africa

Published:2011-02 Issue:3 Volume:193 Page:670-678
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Berg Stefan¹,Garcia-Pelayo M. Carmen¹,Müller Borna²,Hailu Elena³,Asiimwe Benon⁴,Kremer Kristin⁵,Dale James¹,Boniotti M. Beatrice⁶,Rodriguez Sabrina⁷,Hilty Markus⁸,Rigouts Leen⁹,Firdessa Rebuma³,Machado Adelina¹⁰,Mucavele Custodia¹⁰,Ngandolo Bongo Nare Richard¹¹,Bruchfeld Judith¹²,Boschiroli Laura⁶¹³,Müller Annélle²,Sahraoui Naima¹⁴,Pacciarini Maria⁶,Cadmus Simeon¹⁵,Joloba Moses⁴,van Soolingen Dick⁵,Michel Anita L.¹⁶,Djønne Berit¹⁷,Aranaz Alicia⁷,Zinsstag Jakob¹⁸,van Helden Paul²,Portaels Françoise⁹,Kazwala Rudovick¹⁹,Källenius Gunilla²⁰,Hewinson R. Glyn¹,Aseffa Abraham³,Gordon Stephen V.²¹,Smith Noel H.²²

Affiliation:

1. VLA Weybridge, New Haw, Surrey KT15 3NB, United Kingdom

2. Division of Molecular Biology and Human Genetics, Faculty of Health Sciences, Stellenbosch University, P.O. Box 19063, Tygerberg 7505, South Africa

3. Armauer Hansen Research Institute, P.O. Box 1005, Addis Ababa, Ethiopia

4. Department of Medical Microbiology, Makerere University Medical School, P.O. Box 7072, Kampala, Uganda

5. Tuberculosis Reference Laboratory, National Institute for Public Health and the Environment (RIVM), Centre for Infectious Disease Control (CIb/LIS), P.O. Box 1, 3720 BA Bilthoven, Netherlands

6. Reparto Genomica, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia, Via Bianchi n. 9, 25124 Brescia, Italy

7. Dept. de Sanidad Animal, Facultad de Veterinaria, and Centro Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, Avenida, Puerta de Hierro s/n, 28040 Madrid, Spain

8. Institute for Infectious Diseases, University of Bern, Friedbühlstrasse 51, CH-3010 Bern, Switzerland

9. Department of Microbiology, Institute of Tropical Medicine, Nationalestraat 155, B-2000 Antwerp, Belgium

10. Facudade de Veterinaria, Universidade Eduardo Mondlane, CP 257 Maputo, Mozambique

11. Laboratoire de Recherches Vétérinaires et Zootechniques de Farcha, BP 433, N′Djaména, Chad

12. Unit of Infectious Diseases, Department of Medicine, Solna, Karolinska Institutet, Karolinska University Hospital, 17177 Stockholm, Sweden

13. Agence Française de Sécurité Sanitaire des Aliments, 23 Avenue du Général-de-Gaulle, 94706 Maisons-Alfort Cedex, France

14. Université Saad Dahlab, Route de Soumaa, BP 270, Blida, Algeria

15. Department of Veterinary Public Health & Preventive Medicine, University of Ibadan, Ibadan, Nigeria

16. Faculty of Veterinary Science, University of Pretoria, Private Bag X04, and ARC-Onderstepoort Veterinary Institute, Private Bag X05, Onderstepoort 0110, South Africa

17. Department of Animal Health, National Veterinary Institute, BP 750 Sentrum, N-0106 Oslo, Norway

18. Swiss Tropical and Public Health Institute, Socinstrasse 57, 4002 Basel, Switzerland

19. Sokoine University of Agriculture, Morogoro, Tanzania

20. Department of Clinical Science and Education, Karolinska Institutet, Södersjukhuset, 11883 Stockholm, Sweden

21. College of Life Sciences and UCD Conway Institute, University College Dublin, Dublin 4, Ireland

22. VLA Weybridge, New Haw, Surrey KT15 3NB, United Kingdom, and Centre for the Study of Evolution, University of Sussex, Brighton BN1 9QL, United Kingdom

Abstract

ABSTRACT We have identified a clonal complex of Mycobacterium bovis isolated at high frequency from cattle in Uganda, Burundi, Tanzania, and Ethiopia. We have named this related group of M. bovis strains the African 2 (Af2) clonal complex of M. bovis. Af2 strains are defined by a specific chromosomal deletion (RDAf2) and can be identified by the absence of spacers 3 to 7 in their spoligotype patterns. Deletion analysis of M. bovis isolates from Algeria, Mali, Chad, Nigeria, Cameroon, South Africa, and Mozambique did not identify any strains of the Af2 clonal complex, suggesting that this clonal complex of M. bovis is localized in East Africa. The specific spoligotype pattern of the Af2 clonal complex was rarely identified among isolates from outside Africa, and the few isolates that were found and tested were intact at the RDAf2 locus. We conclude that the Af2 clonal complex is localized to cattle in East Africa. We found that strains of the Af2 clonal complex of M. bovis have, in general, four or more copies of the insertion sequence IS 6110 , in contrast to the majority of M. bovis strains isolated from cattle, which are thought to carry only one or a few copies.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.00750-10

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