Evaluation of intraspecies genetic variation within the 16S rRNA gene of Mycoplasma hominis and detection by polymerase chain reaction-Reference-Cited by-同舟云学术

Evaluation of intraspecies genetic variation within the 16S rRNA gene of Mycoplasma hominis and detection by polymerase chain reaction

Published:1993-05 Issue:5 Volume:31 Page:1358-1361
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Blanchard A¹,Yáñez A¹,Dybvig K¹,Watson H L¹,Griffiths G¹,Cassell G H¹

Affiliation:

1. Department of Microbiology, University of Alabama, Birmingham 35294.

Abstract

Mycoplasma hominis is a heterogeneous species with DNA-DNA hybridization values ranging from 51 to 100%. We report here the sequencing of the 16S rRNA gene of a strain (183) that greatly differs from the type strain (PG21) of this species. Comparison of 16S rDNA sequences from these two strains showed limited differences, indicating that the two strains belong to the same rRNA species complex. Using these nucleotide sequence data, we established a rapid method for the detection of M. hominis by using polymerase chain reaction. This method was shown to be sensitive and specific when tested with reference strains and clinical isolates.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.31.5.1358-1361.1993

Reference24 articles.

1. Electrophoretic analysis of proteins from Mycoplasma hominis strains detected by SDS-PAGE, two-dimensional gel electrophoresis and immunoblotting;Andersen H.;J. Gen. Microbiol.,1987

2. Detection and identification of mycoplasmas by amplification of rDNA;Blanchard A.;FEMS Microbiol. Lett.,1991

3. Antigens of Mycoplasma hominis;Brown M. B.;Sex. Transm. Dis.,1983

4. Cassell G. H. and K. B. Waites. 1989. Venereal mycoplasmal infections p. 632-638. In P. D. Hoeprich and M. C. Jordan (ed.) Infectious diseases a modem treatise of infectious processes. J. B. Lippincott Company Philadelphia.

5. Perinatal mycoplasmal infections;Cassell G. H.;Clin. Perinatol.,1991

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