A mec A -Negative Strain of Methicillin-Resistant S taphylococcus aureus with High-Level β-Lactam Resistance Contains Mutations in Three Genes-Reference-Cited by-同舟云学术

A mec A -Negative Strain of Methicillin-Resistant S taphylococcus aureus with High-Level β-Lactam Resistance Contains Mutations in Three Genes

Published:2010-11 Issue:11 Volume:54 Page:4900-4902
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Banerjee Ritu¹,Gretes Michael²,Harlem Christopher³,Basuino Li³,Chambers Henry F.³

Affiliation:

1. Division of Pediatric Infectious Diseases, Mayo Clinic, 200 First Street, Rochester, Minnesota 55905

2. Department of Biochemistry and Molecular Biology, University of British Colombia, Vancouver, Canada

3. Division of Infectious Diseases, San Francisco General Hospital, 1001 Potrero Avenue, San Francisco, California 94110

Abstract

ABSTRACT We previously generated a ceftobiprole-resistant S taphylococcus aureus strain after high inoculum serial passage of a mec A -negative variant of strain COL (R. Banerjee, M. Gretes, L. Basuino, N. Strynadka, and H. F. Chambers, Antimicrob. Agents Chemother. 52:2089-2096, 2008). Genome resequencing of this strain, CRB, revealed that it differs from its parent by five single-nucleotide polymorphisms in three genes, specifically, those encoding PBP4, a low-molecular-weight penicillin-binding protein, GdpP, a predicted signaling protein, and AcrB, a cation multidrug efflux transporter. CRB displayed resistance to a variety of β-lactams but was hypersusceptible to cefoxitin.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.00594-10

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