Affiliation:
1. Immucor, Inc., Norcross, Georgia 30071, USA.
Abstract
A solid-phase erythrocyte adherence assay has been developed for the serological detection of reagin antibodies in syphilis. Capture-S (Immucor, Inc., Norcross, Ga.) is a nontreponemal, qualitative screening test for the detection of immunoglobulin G (IgG) and IgM antilipid antibodies in serum or plasma samples from blood donors. The Capture-S assay utilizes a modified Venereal Disease Research Laboratory antigen bound to microtitration wells and anti-IgG- plus anti-IgM-coated indicator erythrocytes as the detection system. The Capture-S assay was evaluated at six separate sites on 10,942 specimens. For patient samples of clinically diagnosed syphilis categories (n = 366), the Capture-S assay yielded a sensitivity of 80.7% versus 80.3% for the rapid plasma reagin (RPR) card test (Becton Dickinson Microbiology Systems, Cockeysville, Md.). In comparative experiments on patient and donor samples (n = 10,222), the Capture-S assay demonstrated a sensitivity of 94% compared to 91.2% for the RPR card test. The Capture-S and RPR card tests produced essentially equivalent specificities of 99.2% and 99.3%, respectively, for this sample population. For five test sites, the Capture-S and RPR card test demonstrated a 98.3% agreement (10,085 of 10,264) of test results. These evaluations indicate that the Capture-S compares favorably to the RPR card test in assay sensitivity and specificity, with the added benefits of ease of use, accommodation of high-volume testing, and potential for automation.
Publisher
American Society for Microbiology
Reference24 articles.
1. Immunology of syphilis. Annu;Cannefax G. R.;Rev. Med.,1967
2. Center for Disease Control. 1977. Guidelines for evaluation and acceptance of new syphilis serology tests for routine use. U.S. Department of Health Education and Welfare Atlanta Ga.
3. Specific mixed agglutination: mixed erythrocyte-platelet anti-globulin reactions for the detection of platelet antibodies;Coombs R. R. A.;Br. J. Haematol.,1956
4. Galen R. S. and S. R. Gambino. 1975. Beyond normality: the predictive value and efficiency of medical diagnosis p. 10-14. John Wiley & Sons New York.
5. The principle of mixed agglutination applied to tissue culture systems;Högman C.;Vox Sang.,1959
Cited by
4 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献