Affiliation:
1. Department of Agronomy, Ohio State University and Ohio Agricultural Research and Development Center, Wooster, Ohio 44691
Abstract
Nitrate reductase activity was evaluated by four approaches, using four strains of
Rhizobium japonicum
and 11 chlorate-resistant mutants of the four strains. It was concluded that in vitro assays with bacteria or bacteroids provide the most simple and reliable assessment of the presence or absence of nitrate reductase. Nitrite reductase activity with methyl viologen and dithionite was found, but the enzyme activity does not confound the assay of nitrate reductase.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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