Quantitation of Clostridium perfringens Type A Enterotoxin by Electroimmunodiffusion-Reference-Cited by-同舟云学术

Quantitation of Clostridium perfringens Type A Enterotoxin by Electroimmunodiffusion

Published:1972-11 Issue:5 Volume:24 Page:801-804
ISSN:0003-6919
Container-title:Applied Microbiology
language:en
Short-container-title:Appl Microbiol

Author:

Duncan Charles L.¹²,Somers Eileen B.¹²

Affiliation:

1. Food Research Institute, University of Wisconsin, Madison, Wisconsin 53706

2. Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706

Abstract

Conditions for quantitation of Clostridium perfringens type A enterotoxin by electroimmunodiffusion are described. As little as 0.01 μg of enterotoxin could be detected. Electroimmunodiffusion was more sensitive than either single gel diffusion or quantitation based on erythemal activity of the toxin in guinea pig skin.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://journals.asm.org/doi/pdf/10.1128/am.24.5.801-804.1972

Reference8 articles.

1. Ileal loop fluid accumulation and production of diarrhea in rabbits by cell-free products of Clostridium perfringens;Duncan C. L.;J. Bacteriol.,1969

2. Erythemal activity of the cellular enteropathogenic factor of Clostridium perfringens type A;Hauschild A. H. W.;Can. J. Microbiol.,1970

3. Purification and characteristics of the enterotoxin of Clostridium perfringens type A;Hauschild A. H. W.;Can. J. Microbiol.,1971

4. The role of enterotoxin in Clostridium perfringens type A enteritis;Hauschild A. H. W.;Can. J. Microbiol.,1971

5. Studies of electroimmunodiffusion: immunochemical quantitation of proteins in dilute solutions;Lopez M.;Immunochemistry,1969

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1. Enterotoxin synthesis by nonsporulating cultures of Clostridium perfringens;Applied and Environmental Microbiology;1986-09

2. Highly sensitive assay for Clostridium perfringens enterotoxin that uses inhibition of plating efficiency of Vero cells grown in culture;Journal of Clinical Microbiology;1981-05

3. Rapid detection and quantitation of Clostridium perfringens enterostoxin by counterimmunoelectrophoresis;Applied and Environmental Microbiology;1977-08

4. Influence of starch source on sporulation and enterotoxin production by Clostridium perfringens type A;Applied and Environmental Microbiology;1976-03

5. Affinity chromatography purification of Clostridium perfringens enterotoxin;Infection and Immunity;1975-09