Affiliation:
1. Department of Bacteriology, University of Wisconsin, Madison, Wisconsin 53706
Abstract
Three types of evidence are presented to show that the enzymes that hydroxylate nicotinic acid to 2,6-dihydroxynicotinic acid use water as a source of oxygen atoms.
18
O is incorporated into the products from H
2
18
O. Molecular oxygen acts as a terminal electron acceptor, one-half molecule being consumed per molecule of hydroxyl groups incorporated. An external electron acceptor is required for activity in purified preparations.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
31 articles.
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