High-Level Biosynthesis of the Anteiso-C 17 Isoform of the Antibiotic Mycosubtilin in Bacillus subtilis and Characterization of Its Candidacidal Activity-Reference-Cited by-同舟云学术

High-Level Biosynthesis of the Anteiso-C 17 Isoform of the Antibiotic Mycosubtilin in Bacillus subtilis and Characterization of Its Candidacidal Activity

Published:2009-07 Issue:13 Volume:75 Page:4636-4640
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Fickers Patrick¹,Guez Jean-Sébastien²,Damblon Christian³,Leclère Valérie²,Béchet Max²,Jacques Philippe²,Joris Bernard¹

Affiliation:

1. Centre d'Ingénierie des Protéines, Université de Liège, Institut de Chimie, Bat B6, B-4000 Liège, Belgium

2. Laboratoire de Procédés Biologiques, Génie Enzymatique et Microbien (ProBioGEM, UPRES EA 1026), Polytech'Lille, Université des Sciences et Technologies de Lille, F-59655 Villeneuve d'Ascq Cedex, France

3. Chimie Biologique Structurale, Université de Liège, Institut de Chimie, Bat B6, B-4000 Liège, Belgium

Abstract

ABSTRACT High-level production (880 mg liter −1 ) and isolation of the anteiso-C 17 isoform of the lipopeptide mycosubtilin produced by a genetically engineered Bacillus subtilis strain are reported. Antifungal activity of this isoform, as determined via culture and fluorometric and cell leakage assays, suggests its potential therapeutic use as an antifungal agent, in particular against Candida spp.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00548-09

Reference18 articles.

1. Bard, M., A. M. Sturm, C. A. Pierson, S. Brown, K. M. Rogers, S. Nabinger, J. Eckstein, R. Barbuch, N. D. Lees, S. A. Howell, and K. C. Hazen. 2005. Sterol uptake in Candida glabrata: rescue of sterol auxotrophic strains. Diagn. Microbiol. Infect. Dis.52:285-293.

2. Besson, F., F. Peypoux, G. Michel, and L. Delcambe. 1979. Antifungal activity upon Saccharomyces cerevisiae of iturin A, mycosubtilin, bacillomycin L and of their derivatives; inhibition of this antifungal activity by lipid antagonists. J. Antibiot.32:828-833.

3. Besson, F., F. Peypoux, M. J. Quentin, and G. Michel. 1984. Action of antifungal peptidolipids from Bacillus subtilis on the cell membrane of Saccharomyces cerevisiae. J. Antibiot.37:172-177.

4. Reference method for broth dilution antifungal susceptibility testing of yeast. Approved standard M27-A3 2008

5. Dortu, C., P. Fickers, C. Franz, D. Ndagano, M. Huch, W. Holzapfel, B. Joris, and P. Thonart. 2009. Characterisation of an antilisterial bacteriocin produced by Lactobacillus sakei CWBI-B1365 isolated from raw poultry meat and determination of factors controlling its production. Prob. Antimicrob. Pept.1:75-84.

Cited by 50 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Metabolic engineering of “last-line antibiotic” colistin in Paenibacillus polymyxa;Metabolic Engineering;2024-09

2. Engineering of acyl ligase domain in NRPS to design fatty acid moieties of lipopeptides;2024-08-21

3. In Vitro Studies of Endophytic Bacteria Isolated from Ginger (Zingiber officinale) as Potential Plant-Growth-Promoting and Biocontrol Agents against Botrytis cinerea and Colletotrichum acutatum;Plants;2023-11-30

4. Paenibacillus polymyxa Antagonism towards Fusarium: Identification and Optimisation of Antibiotic Production;Toxins;2023-02-09

5. Determination of Taxonomic Affiliation and Assessment of Biotechnological Potential of an Indigenous Strain of Gram-Positive Spore-Forming Bacteria;BIO Web of Conferences;2023