Simplified Microneutralization Test for Serotyping Adenovirus Isolates-Reference-Cited by-同舟云学术

Simplified Microneutralization Test for Serotyping Adenovirus Isolates

Published:2001-08 Issue:8 Volume:39 Page:2984-2986
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Malasig Marietta D.¹,Goswami Pulak R.¹,Crawford-Miksza Leta K.²,Schnurr David P.²,Gray Gregory C.¹

Affiliation:

1. Department of Defense Center for Deployment Health Research, Naval Health Research Center, San Diego,1 and

2. Viral and Rickettsial Disease Laboratory, California Department of Health Services, Richmond,2 California

Abstract

ABSTRACT A simplified microneutralization procedure is described that uses an empirically determined virus challenge dose, a single dilution of antiserum, and observation of cytopathic effect to determine the adenovirus serotype. The simplified test has faster turnaround time and was 96% concordant with a confirmatory test using serial dilutions of type-specific sera. This method will find utility in high-volume serotyping work.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.39.8.2984-2986.2001

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