PCR-Restriction Fragment Length Polymorphism Assay for Detection of gyrA Mutations Associated with Fluoroquinolone Resistance in Campylobacter coli-Reference-Cited by-同舟云学术

PCR-Restriction Fragment Length Polymorphism Assay for Detection of gyrA Mutations Associated with Fluoroquinolone Resistance in Campylobacter coli

Published:2004-12 Issue:12 Volume:48 Page:4886-4888
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Alonso Rodrigo¹,Mateo Estibaliz¹,Girbau Cecilia¹,Churruca Estibaliz¹,Martinez Irati¹,Fernández-Astorga Aurora

Affiliation:

1. Departamento de Inmunología, Microbiología y Parasitología, Facultad de Farmacia, Universidad del País Vasco (UPV-EHU), Vitoria-Gasteiz, Spain

Abstract

ABSTRACT A fragment of the gyrA gene was sequenced from 34 isolates of Campylobacter coli , including 23 isolates resistant to ciprofloxacin. All ciprofloxacin-resistant isolates examined by DNA sequencing carried a point mutation at position Thr-86 on the gyrA gene product, involving the replacement of Thr-86 by Ile. A combined PCR-restriction fragment length polymorphism technique using RsaI was developed to detect this mutation.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.48.12.4886-4888.2004

Reference19 articles.

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