Affiliation:
1. Department of Pharmaceutical Sciences
2. Department of Biomedical Sciences, Division of Experimental and Clinical Microbiology, University of Sassari, Sassari, Italy
Abstract
ABSTRACT
The present study demonstrates the in vitro effectiveness of the macrolide rokitamycin and the phenothiazine compound chlorpromazine against
Acanthamoeba castellanii
. Growth curve evaluations revealed that both drugs inhibit trophozoite growth in dose- and time-dependent ways. The effects of both drugs when they were used at the MICs at which 100% of isolates are inhibited were amoebistatic, but at higher doses they were amoebicidal as well as cysticidal. Experiments showed that when rokitamycin was associated with chlorpromazine or amphotericin B, rokitamycin enhanced their activities. Furthermore, low doses of rokitamycin and chlorpromazine, alone or in combination, blocked the cytopathic effect of
A. castellanii
against WKD cells derived from the human cornea. These results may have important significance in the development of new anti-
Acanthamoeba
compounds.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Reference44 articles.
1. Amaral, L., J. E. Kristiansen, M. Viveiros, and J. Atouguia. 2001. Activity of phenothiazines against antibiotic-resistant Mycobacterium tuberculosis: a review supporting further studies that may elucidate the potential use of thioridazine as anti-tuberculosis therapy. J. Antimicrob. Chemother.47:505-511.
2. Baldessarino R. J. 1980. Drugs and treatment of psychiatric disorders, p. 391-447. In L. S. Goodmann and A. Gilman (ed.), The pharmacological basis of therapeutics, 6th ed. McMillan Publishing Co., New York, N.Y.
3. Viability of pathogenic and nonpathogenic free-living amoebae in long-term storage at a range of temperatures
4. Di Gregorio, C., F. Rivasi, M. Mongiardo, B. De Rienzo, S. Wallace, and G. S. Visvesvara. 1992. Acanthamoeba meningoencephalitis in an AIDS patient: first report from Europe. Arch. Pathol. Lab. Med.116:1363-1365.
5. Activity of phenothiazines against medically important yeasts
Cited by
43 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献