Genomic Identification of Two Phytobacter diazotrophicus Isolates from a Neonatal Intensive Care Unit in Singapore-Reference-Cited by-同舟云学术

Genomic Identification of Two Phytobacter diazotrophicus Isolates from a Neonatal Intensive Care Unit in Singapore

Published:2023-06-20 Issue:6 Volume:12 Page:
ISSN:2576-098X
Container-title:Microbiology Resource Announcements
language:en
Short-container-title:Microbiol Resour Announc

Author:

Hon Peiyun¹,Ko Karrie K. K.²³,Zhong Jonathan C. W.⁴,De Partha P.⁴,Smits Theo H. M.⁵,Low Jiaming⁶,Vasoo Shawn¹⁷⁸⁹,Tsui Clement K. M.¹⁷¹⁰^ORCID

Affiliation:

1. Infectious Diseases Research Laboratory, National Centre for Infectious Diseases, Tan Tock Seng Hospital, Singapore, Singapore

2. Department of Microbiology, Singapore General Hospital, Singapore, Singapore

3. Genome Institute of Singapore, Agency for Science, Technology and Research, Singapore, Singapore

4. Department of Laboratory Medicine, Tan Tock Seng Hospital, Singapore, Singapore

5. Environmental Genomics and Systems Biology Research Group, Institute of Natural Resource Sciences (IUNR), Zurich University of Applied Sciences (ZHAW), Wädenswil, Switzerland

6. Department of Neonatology, National University Hospital, Singapore, Singapore

7. Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, Singapore

8. Department of Infectious Diseases, Tan Tock Seng Hospital, Singapore, Singapore

9. Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore

10. Faculty of Medicine, University of British Columbia, Vancouver, British Columbia, Canada

Abstract

We report the draft genome sequences of two Phytobacter diazotrophicus isolates recovered from a swab specimen from the water faucet located in the Neonatal Intensive Care Unit (ICU), National University Hospital, Singapore. The isolates were misidentified as Cronobacter sakazakii and Klebsiella oxytoca using biochemical methods. Whole-genome sequencing (WGS) was performed to determine their identity.

Funder

Ministry of Health, Singapore

Publisher

American Society for Microbiology

Subject

Genetics,Immunology and Microbiology (miscellaneous),Molecular Biology

Link

https://journals.asm.org/doi/pdf/10.1128/mra.00167-23

Reference12 articles.

1. Resolving taxonomic confusion: establishing the genus Phytobacter on the list of clinically relevant Enterobacteriaceae

2. Emended description of the genus Phytobacter, its type species Phytobacter diazotrophicus (Zhang 2008) and description of Phytobacter ursingii sp. nov.

3. Clinical and Laboratory Standards Institute. 2016. Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria, 3rd ed. Clinical and Laboratory Standards Institute, Wayne, PA.

4. SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing

5. QUAST: quality assessment tool for genome assemblies