Polyvinylpyrrolidone-Agarose Gel Electrophoresis Purification of Polymerase Chain Reaction-Amplifiable DNA from Soils-Reference-Cited by-同舟云学术

Polyvinylpyrrolidone-Agarose Gel Electrophoresis Purification of Polymerase Chain Reaction-Amplifiable DNA from Soils

Published:1993-06 Issue:6 Volume:59 Page:1972-1974
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Young Charles C.¹,Burghoff Robert L.¹,Keim Lois G.¹,Minak-Bernero Vera¹,Lute James R.¹,Hinton Stephen M.¹

Affiliation:

1. Exxon Research and Engineering Company, Route 22 East, Annandale, New Jersey 08801

Abstract

This communication describes a modification of agarose gel electrophoresis to provide a rapid and simple method for the purification of polymerase chain reaction-amplifiable DNA from soil. This modification is to add polyvinylpyrrolidone to the agarose gel. The polyvinylpyrrolidone addition retards the electrophoretic mobility of denaturing phenolic compounds so that they do not comigrate with nucleic acids.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.59.6.1972-1974.1993

Reference14 articles.

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4. Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses;Lane D. J.;Proc. Natl. Acad. Sci. USA,1985

5. Plant phenolic compounds and the isolation of plant enzymes;Loomis W. D.;Phytochemistry,1966

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