Performance of Two Commercial Assays for Detecting Hepatitis E Virus RNA in Acute or Chronic Infections

Author:

Abravanel Florence12,Chapuy-Regaud Sabine12,Lhomme Sébastien12,Dubois Martine12,Peron Jean-Marie3,Alric Laurent4,Rostaing Lionel15,Kamar Nassim15,Izopet Jacques12

Affiliation:

1. INSERM, U1043, Centre de Physiopathologie de Toulouse Purpan, Toulouse, France

2. CHU Toulouse, Hôpital Purpan, Laboratoire de virologie, National Reference Center for Hepatitis E, Toulouse, France

3. CHU Toulouse, Hôpital Purpan, Département de Gastroentérologie, Toulouse, France

4. CHU Toulouse, Hôpital Purpan, Service de médecine interne, Toulouse, France

5. CHU Toulouse, Hôpital Rangueil, Département de Nephrologie, Dialyse et Transplantation multi-organe, Toulouse, France

Abstract

ABSTRACT We assessed the performance of the Ceeram and Altona assays, the first two commercially available hepatitis E virus (HEV) RNA assays, using serial dilutions of 4 HEV-positive reference samples (genotypes 3a, 3c, 3e, and 3f). Both assays provided good analytical sensitivity and high reproducibility for detecting genotype 3 HEV RNA.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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