Direct Whole-Genome Sequencing of Sputum Accurately Identifies Drug-Resistant Mycobacterium tuberculosis Faster than MGIT Culture Sequencing-Reference-Cited by-同舟云学术

Direct Whole-Genome Sequencing of Sputum Accurately Identifies Drug-Resistant Mycobacterium tuberculosis Faster than MGIT Culture Sequencing

Published:2018-08 Issue:8 Volume:56 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Doyle Ronan M.¹²^ORCID,Burgess Carrie¹,Williams Rachel¹,Gorton Rebecca³,Booth Helen⁴⁵,Brown James⁶⁷,Bryant Josephine M.⁸,Chan Jackie⁹,Creer Dean⁶,Holdstock Jolyon⁹,Kunst Heinke¹⁰,Lozewicz Stefan¹¹,Platt Gareth³,Romero Erika Yara¹,Speight Graham⁹,Tiberi Simon¹⁰,Abubakar Ibrahim¹²,Lipman Marc⁶⁷,McHugh Timothy D.³,Breuer Judith¹

Affiliation:

1. Division of Infection and Immunity, University College London, London, United Kingdom

2. Microbiology, Virology and Infection Control, Great Ormond Street Hospital NHS Foundation Trust, London, United Kingdom

3. Centre for Clinical Microbiology, Division of Infection and Immunity, Royal Free Campus, UCL, London, United Kingdom

4. University College London Hospitals NHS Foundation Trust, London, United Kingdom

5. North Central London TB Service-South Hub, Whittington Hospital NHS Trust, London, United Kingdom

6. Royal Free London NHS Foundation Trust, London, United Kingdom

7. UCL Respiratory, Division of Medicine, University College London, London, United Kingdom

8. Molecular Immunity Unit, MRC Laboratory of Molecular Biology, Department of Medicine, University of Cambridge, Cambridge, United Kingdom

9. Oxford Gene Technology, Oxford Begbroke Science Park, Begbroke, Oxfordshire, United Kingdom

10. Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom

11. North Middlesex University Hospital NHS Trust, London, United Kingdom

12. UCL Institute for Global Health, University College London, London, United Kingdom

Abstract

The current methods available to diagnose antimicrobial-resistant Mycobacterium tuberculosis infections require a positive culture or only test a limited number of resistance-associated mutations. A rapid accurate identification of antimicrobial resistance enables the prompt initiation of effective treatment.

Funder

UCLH/UCL NIHR Biomedical Resource Centre

European Union's Seventh Program for Research, Technological Development and Demonstration

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00666-18

Reference34 articles.

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2. Comparison of Xpert MTB/RIF with Other Nucleic Acid Technologies for Diagnosing Pulmonary Tuberculosis in a High HIV Prevalence Setting: A Prospective Study

3. False-positive rifampin resistant results with Xpert MTB/RIF version 4 assay in clinical samples with a low bacterial load

4. Clinical Application of Whole-Genome Sequencing To Inform Treatment for Multidrug-Resistant Tuberculosis Cases

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