Detection of dengue virus type 2 in Aedes albopictus by nucleic acid hybridization with strand-specific RNA probes

Author:

Olson K1,Blair C1,Padmanabhan R1,Beaty B1

Affiliation:

1. Department of Microbiology, Colorado State University, Fort Collins 80523.

Abstract

A molecular hybridization technique with radiolabeled, strand-specific RNA probes was developed to detect dengue virus type 2 RNA in pools of infected Aedes albopictus mosquitoes. One infected mosquito in a pool of 25 could be detected, corresponding to a dengue virus type 2 titer of 2.75 log10 50% tissue culture infectious doses.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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