Affiliation:
1. Department of Microbiology, Colorado State University, Fort Collins 80523.
Abstract
A molecular hybridization technique with radiolabeled, strand-specific RNA probes was developed to detect dengue virus type 2 RNA in pools of infected Aedes albopictus mosquitoes. One infected mosquito in a pool of 25 could be detected, corresponding to a dengue virus type 2 titer of 2.75 log10 50% tissue culture infectious doses.
Publisher
American Society for Microbiology
Reference17 articles.
1. Molecular basis of bunyavirus transmission by mosquitoes: the role of the M RNA segment;Beaty B. J.;Science,1981
2. Genomic sequencing;Church G. M.;Proc. Natl. Acad. Sci. USA,1984
3. Detection of viral nucleic acids by in situ hybridization;Haase A.;Methods Virol.,1984
4. The pathogenesis of dengue. Molecular epidemiology in infectious disease;Halstead S. B.;Am. J. Epidemiol.,1981
5. Detection of dengue virus RNA using nucleic acid hybridization;Henchal E. A.;J. Virol. Methods,1987
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