Affiliation:
1. Department of Virology and Epidemiology, Baylor College of Medicine, Houston, Texas 77025
Abstract
The use of discontinuous counter-immunoelectrophoresis enhanced the reaction between Au/SH antigen and its antibody in agarose. The ionic strength of the Veronal buffer used in the agarose was 0.015 μ, whereas 0.075 μ Veronal (both
p
H 8.6) was used for anode and cathode buffers. Electroendosmosis is increased under such conditions. Au/SH antigen and antibody reacted to give sharp lines within 30 to 45 min as compared with conventional counter-immunoelectrophoresis which required 1 to 3 hr or longer.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Reference3 articles.
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2. Immunoelektrophoretischer Nachweis von"Hepatitis-Associated-Antigen;Pesendorfer F.;(Au/SH Antigen). Klin. Wochenschr.,1970
3. Serum hepatitis antigei (SH): rapid detection by high voltage immunoelectroosmophoresis;Prince A. M.;Science,1969
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