Medium for Toxin Production by Clostridium perfringens in Continuous Culture

Author:

Chou Grace1

Affiliation:

1. Department of Microbiology, University of Hong Kong, Hong Kong

Abstract

A tryptone-salts medium for continuous growth and alpha toxin production of Clostridium perfringens type A in an adapted chemostat is described. In such steady-state cultures, fermentative and biochemical activity of C. perfringens remained unchanged. Toxigenic ability to produce alpha, theta, and nu toxins was preserved.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference10 articles.

1. Methods for the production of the alpha and theta toxins of Clostridium welchii;Adams M. H.;J. Immunol.,1945

2. Factors involved in production of Clostridium welchii alpha toxin;Adams M. H.;J. Exp. Med.,1947

3. Nutritional factors concerned with growth and lecithinase production by Clostridium perfringens;Jayko L. G.;J. Infec. Dis.,1959

4. Production of Clostridiunm perfringenis alpha toxin;Logan M. A.;J. Immunol.,1945

5. Production ofalpha toxin of Clostridium perfringens. I. Preparation of the reproducible peptone medium for the production of the alpha toxin of high potency;Murata R.;Jap. J. Med. Sci. Biol.,1956

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